Aloe Emodin Reduces Cardiac Inflammation Induced by a High-Fat Diet through the TLR4 Signaling Pathway

Figure 1

AE reduces myocardial inflammatory injury in HFD experimental model; HFD also increased the mRNA levels of IL-1β, IL-6, and TNF-α. Staining as described in the Material and Methods section. The frozen heart tissues were sectioned at 5 μm for HE staining (). All images were obtained by an optical microscope with 200x amplification as shown in (a). The representative data of qRT-PCR analysis of RNA extracted from the heart are shown in (b)–(d). All the samples were normalized with GAPDH (). (e, f) The heart tissues in the control group, HFD group, HFD+atorvastatin group (positive control group), and HFD+AE group (the treatment group) were collected and homogenized to detect ICAM-1 and VCAM-1 level by western blot analysis as shown in (e) and (f). GAPDH was used as a loading control. The column figures show the normalized optical density of each protein (; and compared to the ND group; ^#, ^##, and ^### compared to the HFD group).

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