CD154 Induces Interleukin-6 Secretion by Kidney Tubular Epithelial Cells under Hypoxic Conditions: Inhibition by Chloroquine

Figure 7

Chloroquine alleviates the CD154-mediated induction of IL-6 in HK-2 cells. (a) HK-2 cells were preincubated with chloroquine (50 μg/mL) and then incubated for 3 hours at 0.1% O₂ in the presence or not of rsCD154; IL-6 protein was measured by ELISA in the cell culture supernatants (, significant relatively to control, significant relatively to rsCD154 condition). (b) Dose effect experiments: HK-2 cells were preincubated with the indicated concentrations of chloroquine and then incubated for 3 hours at 0.1% O₂ in the presence or not of rsCD154; IL-6 protein was measured by ELISA in the cell culture supernatants (, significant relatively to control, significant relatively to rsCD154 condition). (c) HK-2 cells were incubated for 3 hours at 0.1% O₂ in the presence or not of rsCD154, hydrocortisone (HC) and acetylsalicylic acid (ASA), and IL-6 protein measured by ELISA in cell culture supernatants (, significant relatively to control).

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