Inhibition of GPR91 Reduces Inflammatory Mediators Involved in Active Labor in Myometrium

Figure 1

Expression of GPR91 in myometrium. (a) Human myometrium was obtained from women at term Caesarean section in the absence of labor (term no labor, patients) or from women at term Caesarean section during labor (term in labor, patients). GPR91 mRNA expression was analysed by RT-qPCR, and the fold change was calculated relative to the term no labor group. Individual data points represent different patients, and the horizontal line represents the of each group. , Mann-Whitney test. (b) Myometrium was obtained from mice during LPS-induced labor at 15.5 dpc and time-matched vehicle-injected mice ( mice/group). GPR91 mRNA expression was analysed by RT-qPCR, and the fold change was calculated relative to the vehicle group. Individual data points represent different mice, and the horizontal line represents the of each group. , unpaired -test. (c) Human primary myometrial cells were incubated in the absence or presence of 10 ng/ml IL1B or 10 ng/ml TNF for 5 h or 20 h ( patients). GPR91 mRNA expression was analysed by RT-qPCR, and fold change was calculated relative to basal. Individual data points represent 5 independent experiments, and the horizontal line represents the of each group. , repeated measures one-way ANOVA.

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