Research Article
Advanced Glycated apoA-IV Loses Its Ability to Prevent the LPS-Induced Reduction in Cholesterol Efflux-Related Gene Expression in Macrophages
Figure 2
TNF-alpha and IL-6 secretion and Tnf, Il6, Il1b, and Ccl2 mRNA expression by macrophages treated with unmodified or AGE-apoA-IV and further stimulated with LPS. Bone marrow-derived macrophages (BMDMs) were overloaded with acetylated LDL (50 μg/mL) and incubated with unmodified or AGE-apoA-IV (). Control incubations were kept in DMEM/FAFA alone. After washing, macrophages were stimulated for 24 h with 1 μg/mL of LPS. TNF-alpha and IL-6 secretion were quantified by ELISA (a and b). Gene expression (c–f) was determined by RT-qPCR as described in Material and Methods section. Data are representative of 3 independent experiments (; ).
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