Advanced Glycated apoA-IV Loses Its Ability to Prevent the LPS-Induced Reduction in Cholesterol Efflux-Related Gene Expression in Macrophages

Figure 5

ABCA-1 expression and ¹⁴C-cholesterol efflux in macrophages treated with unmodified and AGE-apoA-IV (a). Bone marrow-derived macrophages (BMDM) were overloaded with acetylated LDL (50 μg/mL) and incubated with unmodified or AGE-apoA-IV for 48 h. After washing, macrophages were stimulated for 24 h with 1 μg/mL of LPS. The ABCA-1 protein level was determined by immunoblot () (b). BMDMs were overloaded with acetylated LDL (50 μg/mL) and ¹⁴C-cholesterol for 48 h. After maintenance in equilibrium medium, cells were incubated with unmodified or AGE-apoA-IV as cholesterol acceptors (). Cholesterol efflux was calculated as ¹⁴C-cholesterol in medium/¹⁴C-cholesterol in medium +¹⁴C-cholesterol in . Basal efflux was determined in incubations with DMEM containing fatty acid-free albumin that was subtracted from those with apoA-IV. Data are representative of 3 independent experiments () and were compared by the Student test.

(a)

(b)