Advanced Glycated apoA-IV Loses Its Ability to Prevent the LPS-Induced Reduction in Cholesterol Efflux-Related Gene Expression in Macrophages

Figure 6

Abca1, Abcg1, Scarb1, Nr1h3, and Nr1h2 mRNA expression in macrophages treated with unmodified or AGE-apoA-IV and further stimulated with LPS. Bone marrow-derived macrophages (BMDMs) were overloaded with acetylated LDL (50 μg/mL) and incubated with unmodified or AGE-apoA-IV. After washing, macrophages were stimulated for 24 h with 1 μg/mL of LPS (). Gene expression was determined by RT-qPCR as described in Material and Methods section. Data are representative of 3 independent experiments (, ) and were compared by ANOVA and Tukey’s post-test.

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