Research Article
Nicotine Impairs the Response of Lung Epithelial Cells to IL-22
Figure 1
Nicotine inhibits the repair capacity of lung epithelial cells. A549 or AECs exposed to nicotine at various concentrations for 72 h were scratched, and the capacity of the cells to fill scratch was determined. Quantitation of percent scratch filled was calculated using the ImageJ software. (a) Scratch repair at 72 h in A549 cells. (b) Quantitation of the same. (c) Micrographs display the scratch repair at 72 h in AECs. (d) Quantitation of the same. Data is presented as the of biological replicates of for A549 and for AECs. Arrows point to the scratches. Scale for micrograph is 20 μm.
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