Nicotine Impairs the Response of Lung Epithelial Cells to IL-22
Nicotine inhibits the repair capacity of lung epithelial cells. A549 or AECs exposed to nicotine at various concentrations for 72 h were scratched, and the capacity of the cells to fill scratch was determined. Quantitation of percent scratch filled was calculated using the ImageJ software. (a) Scratch repair at 72 h in A549 cells. (b) Quantitation of the same. (c) Micrographs display the scratch repair at 72 h in AECs. (d) Quantitation of the same. Data is presented as the of biological replicates of for A549 and for AECs. Arrows point to the scratches. Scale for micrograph is 20 μm.
Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.