Advanced Glycation End Products Induce Vascular Smooth Muscle Cell-Derived Foam Cell Formation and Transdifferentiate to a Macrophage-Like State

Figure 3

RAGE is essential for CML-induced lipid accumulation in VSMCs. Predesigned siRNAs for LOX-1 or RAGE were transfected to primary VSMCs at 25 nmol/l. VSMCs were used for experiments 72 h posttransfection and then treated by oxLDL or oxLDL and CML. (a) Representative western blot images of LOX-1. Results are representative of 3 experiments with similar results. . (b) Oil red O staining of primary VSMCs transfected RAGE siRNA or negative control. Magnification ×200. . (c) Representative western blot images of LOX-1. Results are representative of 3 experiments with similar results. . (d) Oil red O staining of primary VSMCs transfected LOX-1 siRNA or negative control. Magnification ×200. .

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