Chinese Medicine Huzhen Tongfeng Formula Effectively Attenuates Gouty Arthritis by Inhibiting Arachidonic Acid Metabolism and Inflammatory Mediators

Figure 5

Effects of HZTF on LPS-induced ROS accumulation in RAW264.7 cells. After pretreatment with HZTF (12.5, 25, and 50 μg/mL) or positive drug (dexamethasone at 10 μg/mL) treatment for 2 h then treatment with LPS (100 ng/mL) for 24 h, cells were incubated with 10 μM DCFH-DA for 30 min. (a) The fluorescent microscopy showing production of intracellular ROS (scale bar: 50 μm). (b) The relative fluorescence intensity was analyzed by the Image J software (version 1.51). Data were presented as the (). ^###, compared with the control group; , , compared with the LPS group.

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