Corticosterone Inhibits LPS-Induced NLRP3 Inflammasome Priming in Macrophages by Suppressing Xanthine Oxidase

Figure 6

Supernatant levels of IL-1β and IL-18 in ASC-reconstituted RAW264.7 cells treated with the combination of different drugs. The control group received no treatment. In the LPS group, cells were stimulated with LPS (1 μg/ml) for 2 h. In the CORT group, cells were pretreated with CORT (700 ng/ml) for 1 h and stimulated with LPS (1 μg/ml) for 2 h. In the allopurinol group, cells were pretreated with allopurinol (250 μg/ml) for 1 h and stimulated with LPS (1 μg/ml) for 2 h. In the CORT group, cells were pretreated with RU486 (1 μM) for 1 h, then treated with CORT (700 ng/ml) for an additional 1 h, and were finally stimulated with LPS (1 μg/ml) for 2 h. After the cells were pretreated with different drugs and primed with LPS, ATP was applied for 1 h in all groups. (a) Supernatant levels of IL-1β. (b) Supernatant levels of IL-18. Values are mean (s.d.) (). , versus the LPS group; ^# versus the CORT group.

(a)

(b)