Dexmedetomidine Attenuates Monocyte-Endothelial Adherence via Inhibiting Connexin43 on Vascular Endothelial Cells

Figure 4

Dexmedetomidine attenuated U937-HUVEC adhesion, as well as the contents of MCP-1, sICAM-1, and sVCAM-1. (a) Effects of dexmedetomidine (Dexm: 0.1 nM and 1 nM, 24 hours) on the activation of MAPKs (p-ERK/ERK, p-p38/p38, and p-JNK/JNK) (, vs. control). (b–d) The contents of MCP-1, sICAM-1, and sVCAM-1 when HUVECs were pretreated with dexmedetomidine (Dexm: 0.1 nM and 1 nM, 24 hours) (, vs. control; ^# vs. 0.1 nM group). (e) The changes of U937-HUVEC adhesion when HUVECs were pretreated with dexmedetomidine (Dexm: 0.1 nM and 1 nM, 24 hours) (, vs. control; ^# vs. 0.1 nM group). In all experiments, HUVECs were all pretreated with TNF-α (10 ng/ml, 12 h). Effects of TNF-α are showed in Supplemental Figure 1.

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