Association of Pathogenic Th17 Cells with the Disease Severity and Its Potential Implication for Biological Treatment Selection in Psoriasis Patients

Figure 6

Determination of the pathogenic phenotype of Th17 cells in a psoriasis patient is useful to choose the biological treatment. (a) Prior to treatment, an integral analysis of Th17 cells was performed in patient P52 (PASI 18.3). The photograph shows the most significant psoriatic lesion. Microscopy images show the evaluation of Th17 cells in the lesional skin using antibodies anti-CD4 (green), anti-IFN-γ (cyan), anti-IL-17 or anti-CD161 (red), and nuclei (blue). Flow cytometry analyses of CD161 and RORγt expression in CD4 cells and production of IL-17 and IFN-γ in Th17 cells after stimulation. (b) Psoriasis patient photograph after adalimumab administration (PASI 0). Evaluation of IL-17 and IFN-γ expression in Th17 cells by flow cytometry (bottom plot). Scale  μm.

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