Contribution of Connexin Hemichannels to the Pathogenesis of Acute Lung Injury

Figure 3

P5 inhibited the extracellular release of HMGB1 in vitro and in vivo. (a) Cultured HPMVEC were divided into Cont, P5 (20 μg/ml), LPS (0.5 μg/ml), and LPS+P5 (0.5 μg/ml LPS and 20 μg/ml P5) groups. At 16 hr post-LPS challenge, culture medium was collected, concentrated, and subjected to Western blotting analysis of HMGB1. . (b) Balb/c mice were divided into 4 groups: Sham (surgery+saline), P5 (8 mg/kg P5), ALI (5 mg/kg LPS+saline), and ALI+P5 (5 mg/kg P5). ALI was induced by intratracheal instillation of LPS. Saline or P5 was administered twice through ip at 2 and 16 hr post-ALI induction. BALF was collected at 4 hr after second saline or P5 administration, concentrated, and subjected to Western blotting analysis of HMGB1. a.u.: arbitrary unit; .

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