ASK1 Enhances Angiotensin II-Induced Liver Fibrosis In Vitro by Mediating Endoplasmic Reticulum Stress-Dependent Exosomes

Figure 6

Effects of an ASK1 inhibitor on Ang II-mediated ERS and proinflammatory phenomena in LX-2 cells. LX-2 cells were treated with Ang II alone or cotreated with Ang II and an ASK1 inhibitor (GS-4997). (a) The protein bands of p-ASK1, ASK1, GRP78, PERK, p-PERK, and CHOP are shown in the left panel, and the corresponding protein levels are shown in the right panel. (b) The ROS levels measured using flow cytometry are shown in the left panel, and quantified results for ROS generation are depicted in the right panel. ELISA was used to determine the expression levels of proinflammatory cytokines, including IL-1β (c), IL-18 (d), and TNF-α (e). Results are expressed as the (SD). , , and vs. blank. ^# vs. Ang II.

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