miR-199a-5p Exacerbated Intestinal Barrier Dysfunction through Inhibiting Surfactant Protein D and Activating NF-κB Pathway in Sepsis

Figure 7

Inhibition of miR-199a-5p contributed to IECs injury via targeting SP-D and inactivating NF-κB pathway. (a, b) SP-D expression in IECs. IECs were transfected with SP-D siRNA for 48 hours. (c) IEC apoptosis was evaluated by flow cytometry. IECs were transfected with SP-D siRNA and then transfected with miR-199a-5p inhibitor. (d) Protein levels of ZO-1, p-NF-κB, and total-NF-κB in ECs. Three independent experiments were carried out. Error bars stand for the of at least triplicate experiments. .

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