Sustained Immunoparalysis in Endotoxin-Tolerized Monocytic Cells

Figure 4

LPS-tolerized (1 μg/ml) THP-1 monocytes (10⁶ cells/ml) demonstrate sustained cytokine mRNA and protein alterations upon 6 h LPS restimulation (1 μg/ml) compared to previously untreated cells. mRNA transcription (a) and protein secretion (b) of TNFα and IL-1β were measured at different time points after 48 h preincubation followed by resting durations of 0 d (≙ passage (P)1), 2 d (≙ P2), and 5 d (≙ P3) prior to repeated LPS exposure. ; .

(a)

(b)