Proinflammatory Effects of Ubiquitin-Specific Protease 5 (USP5) in Rheumatoid Arthritis Fibroblast-Like Synoviocytes

Figure 4

USP5 promoted NF-κB signaling activation in IL-1β-treated RA-FLS. (a) Phosphorylation levels of IκBα and p65 in USP5-overexpressed RA-FLS after IL-1β (10 ng/ml) treatment for 9 hours. (b) Quantification of phosphorylation of IκBα and p65 in (a). (c) Phosphorylation levels of IκBα and p65 in USP5-silenced RA-FLS after IL-1β (10 ng/ml) treatment for 9 hours. (d) Quantification of phosphorylation of IκBα and p65 in (c). (e, f) After infection with lentivirus containing USP5 expression plasmid (e) or USP5 shRNA plasmid (f) for 48 hours, RA-FLS were transfected with NF-κB reporter plasmid and phRL-TK plasmid (internal control) for 24 hours, followed by the stimulation with IL-1β (10 ng/ml) for 9 hours; the activation of the NF-κB promoter was measured by a dual-luciferase reporter gene assay. , , and .

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