Plasma and Cellular Forms of Fibronectin as Prognostic Markers in Sepsis

Figure 3

Representative immunopatterns of FN-fs (a) and FN-fibrin complexes (b) in plasma samples of patients with sepsis and control group. The plasma samples of sepsis patients and normal individuals were subjected to SDS (10%)-polyacrylamide gel (a) and SDS-agarose immunoblotting under nonreducing conditions (b). For details, see Materials and Method. The molecular weight of the FN band (a) was estimated using protein standard. Plasma samples (a) lane 1, control; lane 2, sepsis patient; (b) lanes 1-2, sepsis patients; lane 3, control. The molecular masses of the 750 to 1600 kDa plasma FN-fibrin complexes, 500 kDa FN dimer, and FN monomer are shown by arrows on the left.

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