Paeonol Attenuates Methotrexate-Induced Cardiac Toxicity in Rats by Inhibiting Oxidative Stress and Suppressing TLR4-Induced NF-κB Inflammatory Pathway

Figure 3

Effect of paeonol (PAE) on cardiac nuclear factor- (NF-) κB/p65 immunostaining in methotrexate- (MTX-) induced cardiac toxicity in rats. (a) Control group showing positive expression in the cytoplasm of scattered cells of the endomysium (arrow). Notice negative expression in the endothelium (arrowhead). (b) MTX-treated group showing positive cytoplasmic and nuclear expression in many cells (arrows) and high expression in the endothelium (arrowhead). (c) PAE+MTX group showing positive expression in scattered cells (arrow) and low expression in the endothelium (arrowhead). (d) Semiquantitative analysis of the results. Values represent . “A” means significant difference compared to the control group and “B” means significant difference compared to the MTX-treated group, at .

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