Response of Human Mesenchymal Stromal Cells from Periodontal Tissue to LPS Depends on the Purity but Not on the LPS Source

Figure 6

Effect of the TLR4 inhibitor TAK-242 on the gene expression levels of IL-6, IL-8, and MCP-1 in the hPDLSC response to different LPS preparations. Primary hPDLSCs were stimulated with commercially available “standard” P. gingivalis LPS (1 μg/ml), “ultrapure” P. gingivalis LPS (1 μg/ml), and “ultrapure” E. coli LPS (1 μg/ml) in the presence (250 ng/ml) of sCD14. Additionally, in the appropriate groups, TLR4 inhibitor TAK-242 (5 μM) was added for 24 h. The resulting gene expression levels of IL-6, IL-8, and MCP-1 were measured by qPCR. The -axis represents the -fold expression levels of target gene in relation to unstimulated cells (). Data are presented as of five different donors. Significantly different vs. control; ^#significantly different vs. stimulation in the absence of TAK-242.