Therapeutic Effect of Ginsenoside Rd on Experimental Autoimmune Encephalomyelitis Model Mice: Regulation of Inflammation and Treg/Th17 Cell Balance

Figure 2

Ginsenoside Rd treatment ameliorated the severity of EAE via inhibiting spinal cord inflammation and demyelination. Following immunization and treatment with Ginsenoside Rd, some mice from the control, EAE, and Ginsenoside Rd-treated EAE groups were subjected to histopathological assay at day 20 postimmunization. Spinal cord tissue sections were stained with H&E or LBP, respectively. In addition, the degrees of inflammatory infiltrates and demyelination were semiquantitatively analyzed. Data are expressed as the for each group (). (a) H&E analysis of the spinal cord sections. Magnification ×40, 200. (b) LBP analysis of the spinal cord sections. Magnification ×40, 200. (c) MBP immunofluorescence staining of the spinal cord sections. Tissue sections of spinal cord from each group mice were stained with antibodies against MBP (red) and DAPI (blue, nucleus). Magnification ×50, 200. , vs. EAE group. Gin Rd: Ginsenoside Rd.