To study the effect of vitamin D supplementation on the whole genome gene expression in the skeletal muscle.
Vitamin D deficient frail older adults. Calcifediol supplementation group () & placebo group ()
10 μg per day calcifediol for 6 months
Affymetrix HuGene 2.1ST arrays were used for the whole genome gene expression profiling of muscle biopsies obtained before and after 6 months of intervention for all subjects.
No significant effect of supplementation was seen on the skeletal muscle transcriptome of frail older adults.
To study the effect of vitamin D supplementation on transcriptome.
Obese subjects () and normal weight subjects () each randomized into supplementation and placebo groups.
50 μg (2000 IU) daily dose of vitamin D for 12 weeks
Data were collected at baseline, 6 and 12 weeks from all subjects. The peripheral blood gene expression was analysed using GlobinLock oligonucleotides followed by RNA sequencing.
Vitamin D supplementation affected the gene expression in obese subjects but not in normal weight subjects.
To identify long-term supplementation effects of folic acid and vitamin B12 on genome wide DNA methylation.
87 subjects with mildly elevated homocysteine levels ( folic acid and B12 group, placebo group)
400 μg folic acid and 500 μg vitamin B12 per day for 2 years
Infinium HumanMethylation450 BeadChip was used for genome-wide DNA methylation, and DNA samples were collected before and after intervention from all subjects.
Long-term folic acid and B12 supplementation have effects on DNA methylation of genes including those implicated in the developmental processes.
To study the effect of vitamin D supplementation on the gene expression and plasma cytokine levels.
305 community-dwelling individuals aged 65 years and above
2 treatment groups with 4000 IU & 2000 IU vitamin D3 supplementation, respectively, for 12 months and one placebo group.
Genome-wide genotypes were measure at baseline, and transcriptome and plasma cytokine levels were measured at baseline and after 12 months of intervention.
No significant effect of high dose vitamin D supplementation was observed on the gene expression and concentration of selected cytokine levels.
To analyze the effects of vitamin D supplementation on the gene expression
Vitamin D supplementation group () and placebo group ()
20,000 IU dose of vitamin D per week for 3 to 5 years
Blood samples were drawn for preparation of RNA, and microarray analysis was used to determine the mRNA gene expression in the blood.
Between the two groups, no significant changes in the gene expression were found after supplementation. On analyzing separately based on gender, women showed significant changes on the gene expression. In total, 99 genes were found to be regulated.
To determine the effects of vitamin A supplementation on gene expression cytokines secreted by TCD4+ lymphocytes.
Vitamin A supplementation group of atherosclerotic patients (), atherosclerotic patients receiving placebo group (), healthy subjects receiving vitamin A supplementation ()
25000 IU retinyl palmitate per day for 4 months.
Fasting blood samples were collected before and after 4 months from all subjects. Gene expression pattern of relevant cytokines of CD4+ T cells including was determined by real-time PCR.
Reduced gene expression of IFN-γ and T-bet in all patients after supplementation. Increased gene expression of IL-4 in subjects who received supplementation. Positive role of vitamin A supplementation on the gene expression.
