Mediators of Inflammation / 2020 / Article / Fig 5

Research Article

Salvianolic Acid D Alleviates Cerebral Ischemia-Reperfusion Injury by Suppressing the Cytoplasmic Translocation and Release of HMGB1-Triggered NF-κB Activation to Inhibit Inflammatory Response

Figure 5

SalD inhibited HMGB1-triggered NF-κB activation in OGD/R-injured PC12 cells. (a) The relative intensity of TLR4 (). (b) The relative intensity of MyD88 (). (c) The relative intensity of TRAF6 (). (d) The relative p-NF-κB p65/NF-κB p65 ratio (). (e) The relative intensity of cytoplasmic HMGB1 (). (f) The relative intensity of nuclear HMGB1 (). (g) The immunofluorescence image of HMGB1 and p-NF-κB p65 double staining in PC12 cells. Proteins were acquired from PC12 cells and assayed by the Western blot method. Nuclear proteins were normalized to the intensity of Lamin B1, while other proteins were normalized to the intensity of β-actin. Data were expressed as . ## versus the control group; and versus the OGD/R group. .

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