Gene Expression Profiling of Mediators Associated with the Inflammatory Pathways in the Intestinal Tissue from Patients with Ulcerative Colitis

Table 1

Oligonucleotide sequences for real-time RT-PCR.

Gene	Gene bank	Left	Right	UPL (Universal Probe Library)
AGR2	NM_006408.3	ggtgggtgaggaaatccag	gtaggagagggccacaagg	47
IRGM	NM_001145805.1	gcttgaaaaagagcagagcatt	gggcccaactgaagtgag	63
ORMDL3	NM_139280.1	tcaccaacctcattcacaaca	caaagggtgtccccttcac	4
XBP1	NM_005080.3, NM_001079539.1	ggagttaagacagcgcttgg	cactggcctcacttcattcc	37
HSPA5	NM_005347.4	aaaagcatttgggcagacc	ttaatggctgtattgggcttg	54
UBD	NM_006398.3	agagatggctcccaatgct	ggcatcaaaggtcattaaatcc	34
UBE2L3	NM_003347.2	atgcattctggggaaggag	ttgcggatttcttcaagctc	75
CUL2	NM_003591.3, NM_001198777.1, NM_001198778.1, NM_001198779.1	gaatgctgctccgagaaatc	ccccatggattactttctgg	22
FOXC2	NM_005251.2	ggggacctgaaccacctc	aacatctcccgcacgttg	3
CYLD	NM_001042412.1, NM_001042355.1, NM_015247.2	cagtctccggaatattctttgg	cagtgaaaccttgaccacga	11
FOXO4	NM_005938.3, NM_001170931.1	cgagggactggacttcaact	ggctcaagggtaaagagtagatatga	36
TNFRSF14	NM_003820.2	ctttgcctggacagctcct	cagcagagaggggctcag	3
LAMP3	NM_014398.3	tgctcatttttatgggattgc	tgagtttatttgatgccttcatctt	26
FCGR2A	NM_021642.3, NM_001136219.1	cctgtgaccatcactgtcca	agccacaatgatccccatt	44
LSP1	NM_001242932.1; NM_002339.2 NM_001013253.1 NM_001013254.1 NM_001013255.1	tccctaggcgtcccatct	ggcaacaggaagcaacttct	81
CTLA4	NM_005214.4 NM_001037631.2	ttcatccctgtcttctgcaa	agtggctttgcctggagat	40
HSP90B1	NM_003299.1	tcctatttatgtatggagcagcaa	gcagcttcatcatcagattcttc	69
DOK3	NM_024872.2 NM_001144875.1; NM_001144876.1	tccccatggaggaaaactc	caccaccacgggaaactc	63
SNX20	NM_001144972.1 NM_153337.2	cacacccggactgatgtgt	tctctgttagtggtgcctgga	76
SLC26A3	NM_000111.2	ccatcatcgtgctgattgtc	agctgccaggacggactt	2
SLC39A4	NM_017767.2 NM_130849.2	gctccagtgtgtgggaca	gcctgttccgacagtcca	46
FOLR1	NM_016729.2 NM_000802.3 NM_016725.2 NM_016724.2	gaggacaagttgcatgagca	cctggctggtgttggtaga	65
SOD2	NM_001024465.1; NM_001024466.1	aatcaggatccactgcaagg	taagcgtgctcccacacat	3
TDO2	NM_005651.2	ggagaagaaaatgaactgctacttaaa	ggctctaaacctggagttctttc	15
ALDOB	NM_000035.3	cggccaaaggacagtatgtt	aagagcgactgggtggaa	45
GAPDH	NM_002046.3	agccacatcgctcagacac	gcccaatacgaccaaatcc	60

Assays were designed to detect both transcript isoforms. UPL: Universal Probe Library.