Neutrophil Extracellular Traps: Signaling Properties and Disease Relevance

Table 1

The detection targets and methods of NETs in cells, tissues, and blood.

Sample type	Detection target	Detection method	Advantage	Limitations
Cell or tissue	dsDNA	SYTOX Green nucleic acid dye	Simple and direct	Less specific
	DNA, citH3, and MPO (or NE)	Immunofluorescence	Specific and widely accepted	Qualitative rather than quantitative
Blood	dsDNA	SYTOX Green nucleic acid dye	Simple and easy	Less specific
	dsDNA, nucleosomes, MPO-DNA complexes (MPO or NE), and citH3 (citH4)	ELISA	Specific and widely accepted	—