Mediators of Inflammation / 2020 / Article / Fig 2

Research Article

Luteolin Attenuates IL-1β-Induced THP-1 Adhesion to ARPE-19 Cells via Suppression of NF-κB and MAPK Pathways

Figure 2

Luteolin inhibited inflammatory mediator expression and increased anti-inflammatory protein HO-1 expression in IL-1β-stimulated ARPE19 cells. (a) Cell viability of ARPE19 cells treated with various luteolin (LU) concentrations (0–100 μM). Cells were pretreated with luteolin for 1 h, then stimulated with IL-1β (1 ng/mL) for 24 h. (b) Cells were pretreated with different LU doses and then incubated with IL-1β (1 ng/mL) for 30 min or 24 h. Western blots show iNOS protein expression. (c) The fold-change in iNOS protein expression was measured relative to β-actin expression. (d) Western blots show HO-1 protein expression. (e) The fold-change in HO-1 protein expression was measured relative to β-actin expression. (f) Western blots show phosphorylated AKT protein expression. (g) The fold-change in pAKT protein expression was measured relative to AKT expression. Data represent the . , , compared to ARPE-19 cells stimulated with IL-1β alone.
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