Mediators of Inflammation / 2020 / Article / Fig 3

Research Article

Luteolin Attenuates IL-1β-Induced THP-1 Adhesion to ARPE-19 Cells via Suppression of NF-κB and MAPK Pathways

Figure 3

Luteolin inhibited inflammation-related cytokine expression and attenuated THP-1 cell adherence to IL-1β-stimulated ARPE-19 cells. Cells were pretreated with different doses of luteolin (LU) and then incubated with IL-1β (1 ng/mL) for 24 h. ELISA results showed the levels of (a) IL-6, (b) IL-8, (c) sICAM-1, and (d) MCP-1. (e) LU significantly suppressed THP-1 cell adherence to IL-1β-stimulated ARPE-19 cells. Fluorescent-labeled THP-1 cells (green) were cocultured with control (CTL) or IL-1β-stimulated ARPE-19 cells in the absence or presence of the indicated LU concentrations. (f) The fluorescence intensity revealed THP-1 cell adhesion to IL-1β-stimulated ARPE-19 cells, which was used to quantify calcein-AM fluorescence. The data represent the . , , compared to ARPE-19 cells stimulated with IL-1β alone.

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