Autophagy Contributes to Host Immunity and Protection against Zika Virus Infection via Type I IFN Signaling

Figure 6

(a) GSE97919 Zika data set. GSEA (gene set enrichment analysis) shows there was a positive correlation between ZIKV infection and IFN-α pathway activation. . (b) Different genes enriched by IFN-α activation upon ZIKV infection, and 70 IFN-α pathway genes were upregulated. (c) Analysis of interaction among IFN-α pathway, negative regulation of autophagy, and positive regulation of autophagy. (d) RAW264.7 cells were transfected with IFNAR1 siRNA, IFNAR2 siRNA, IFNGR1 CRISPR-Cas9, IFNGR2 siRNA, IFNLR1 siRNA, or IL10R2 siRNA, respectively. qRT-PCR was performed to test the knockdown or knockout efficiency. (e) Cells above were cultured and transfected with LC3-GFP, then infected with ZIKV, . LC3 fluoresce was observed using confocal microscopy. (f) LC3 puncta numbers in 100 cells of each sample were counted. . Data were representative of three experiment results.

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