lncRNA IGHCγ1 Acts as a ceRNA to Regulate Macrophage Inflammation via the miR-6891-3p/TLR4 Axis in Osteoarthritis

Figure 5

miR-6891-3p regulated in a targeted manner TLR4 in macrophages. (a) The binding sequence of TLR4 3UTR recognized by miR-6891-3p (information acquired in TargetScan database). (b) miR-6891-3p inhibitors promoted the mRNA expression of TLR4 demonstrated by real-time PCR (; ). (c) miR-6891-3p inhibitors promoted TLR4 expression demonstrated by western blot (representative figure for western blot). (d) TLR4 protein expression demonstrated by densitometry of the western blot (; ). (e) The luciferase reporter assay demonstrated the luciferase activity of WT TLR4 3UTR was significantly restrained by miR-6891-3p mimics but not miR-6891-3p inhibitors (; ; ).

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