lncRNA IGHCγ1 Acts as a ceRNA to Regulate Macrophage Inflammation via the miR-6891-3p/TLR4 Axis in Osteoarthritis
lncRNA IGHCγ1 regulated TLR4 expression as a ceRNA of miR-6891-3p in macrophages. (a) Upregulation of TLR4 significantly enhanced the proliferation of macrophages at 48 h and 72 h demonstrated by CCK-8 (;;). (b) Overexpression of TLR4 promoted IL-6 and TNF-α mRNAs in macrophages (;;). (c) Overexpression of TLR4 promoted IL-6 and TNF-α production in the supernatant of macrophages (;). (d) The upregulation of TLR4 mRNA by lncRNA IGHCγ1 was significantly suppressed by miR-6891-3p mimics as demonstrated by real-time PCR (; compared with the -/- group, ; in contrast to the +/- group, ##). (e) The upregulation of TLR4 protein by lncRNA IGHCγ1 was significantly inhibited by miR-6891-3p mimics as demonstrated by western blot (representative picture for western blot). (f) The densitometry of TLR4 protein detected by western blot (; compared with the -/- group, ; compared with the +/- group, #).
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