miR-6869-5p Inhibits Glioma Cell Proliferation and Invasion via Targeting PGK1

Figure 3

miR-6869-5p targetedly regulated PGK1 and affected cell proliferation and invasion of glioma cells. (a) Base complementary sequence between 3UTR of PGK1 and miR-6869-5p. (b) miR-6869-5p mimics inhibited both mRNA and protein expression of PGK1 (; ; representative blotting images). (c) miR-6869-5p inhibitors promoted both mRNA and protein expression of PGK1 (; ; representative blotting images). (d) The luciferase reporter assay demonstrated that miR-6869-5p could regulate PGK1 expression (; ). (e) CCK-8 assay demonstrated that miR-6869-5p inhibited the proliferation of U87 glioma cells via targeting PGK1 (; vs. the control group, ; vs. the Lv-PGK1 group, ^#; ^##). (f) Representative figures for the scratch test at 0 h and 48 h (three independent experiments). (g) Transwell assay showed miR-6869-5p mimics inhibited U87 cell invasion by targeting PGK1 (; vs. the control group, ; vs. the Lv-PGK1 group, ^##).

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