Characterization of Transverse Aortic Constriction in Mice Based on the Specific Recruitment of Leukocytes to the Hypertrophic Myocardium and the Aorta Ascendens

Figure 2

Innate immune response in the myocardium and the ascending and descending parts of the aorta. (a)–(l) The CD45⁺ immune cells, neutrophils, Ly6C^low, and Ly6C^high macrophages in the tissue of the LV, the aorta ascendens and descendens of the mice were quantified via flow cytometry. The particular cell subsets were defined as follows: neutrophils as CD45⁺ F4/80^- Ly6G⁺ and macrophages as CD45⁺ F4/80⁺ Ly6G^-. Moreover, the Ly6C^high and Ly6C^low macrophages were further discriminated according to the respective Ly6C surface expression. The above individual columns indicate the significant differences between the TAC and respective sham group; , , , and .