Characterization of Transverse Aortic Constriction in Mice Based on the Specific Recruitment of Leukocytes to the Hypertrophic Myocardium and the Aorta Ascendens

Figure 3

Innate immune response in the spleen. (a)–(e) The CD45⁺ immune cells, neutrophils, and Ly6C^low and Ly6C^high macrophages, and DCs in the spleen of the mice were quantified via flow cytometry. The particular cell subsets were defined as follows: neutrophils as CD45⁺ F4/80^- Ly6G⁺ and macrophages as CD45⁺ F4/80⁺ Ly6G^-. Moreover, the Ly6C^high and Ly6C^low macrophages were further discriminated according to the respective Ly6C surface expression, and the DCs were defined as CD45⁺ F4/80^- Ly6G^-and CD11c⁺. The above individual columns indicate the significant differences between the TAC and respective sham group; , and .

(a)

(b)

(c)

(d)

(e)