Characterization of Transverse Aortic Constriction in Mice Based on the Specific Recruitment of Leukocytes to the Hypertrophic Myocardium and the Aorta Ascendens

Figure 4

Innate immune cell quantification and cytokine levels in response to TAC in the blood. (a)–(d) The CD45⁺ immune cells, neutrophils, and Ly6C^low and Ly6C^high monocytes in the blood of the mice were quantified using flow cytometry. The particular cell subsets were defined as follows: neutrophils as CD45⁺ CD115^- Ly6G⁺ and monocytes as CD45⁺ CD115⁺ Ly6G^-. In addition, the Ly6C^high and Ly6C^low monocytes were further discriminated according to the respective Ly6C surface expression. (e)–(g) The cytokine levels of IL-6, IL-1β, and TNF-α in the serum of the mice were measured at days 3 and 6 after TAC or after day 3 after sham surgery. The above individual columns indicate the significant differences between the TAC and respective sham group; , , and .

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