lncRNA PAPPA-AS1 Induces the Development of Hypertrophic Scar by Upregulating TLR4 through Interacting with TAF15

Figure 6

lncRNA PAPPA-AS1 induced the transcription of TLR4 by recruiting TAF15 to the TLR4 promoter. (a) The relative expression of PAPPA-AS1 was detected by qRT-PCR. (b) qRT-PCR was used to detect the expression of TAF15 ( vs. ). (c) The expression levels of TAF15 and TLR4 in the tissues were evaluated by RT-qPCR ( vs. normal tissues). (d) The correlation between the expression of TAF15 and TLR4 in the HTS tissues was evaluated. (e) RT-qPCR was used to detect the expression of TAF15 and TLR4 in the treated HTsFb cells ( vs. ). (f) ChIP assay evaluated the interaction between TAF15 and the promoter of TLR4 ( vs. IgG). (g) The interaction between TAF15 and the promoter of TLR4 was verified by luciferase assay. Control group: normally cultured HTsFb cells; group: HTsFb cells were transfected with sh-NC plasmid and pcDNA3.1-NC plasmid simultaneously; sh-PAPPA-AS1#1 group: HTsFb cells were transfected with sh-PAPPA-AS1#1 plasmid; pcDNA3.1-PAPPA-AS1 group: HTsFb cells were transfected with pcDNA3.1-PAPPA-AS1 plasmid; normal tissue group: human normal skin tissue; scar tissue: human scar tissue; group: HTsFb cells were transfected with sh-NC plasmid and over-NC plasmid simultaneously; sh-TAF15 group: HTsFb cells were transfected with sh-TAF15 plasmid; over-TAF15 group: HTsFb cells were transfected with over-TAF15 plasmid. ( vs. NC).

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