lncRNA PAPPA-AS1 Induces the Development of Hypertrophic Scar by Upregulating TLR4 through Interacting with TAF15

Figure 7

PAPPA-AS1 promoted the proliferation of HTsFb cells by regulating the TAF15/TLR4 axis. (a) The relative expression of TLR4 was detected by qRT-PCR. (b) MTT assay was used to detect the proliferation of the treated HTsFb cells (, vs. sh-NC; vs. sh-PAPPA-AS1#1). (c) The expression levels of TLR4, MyD88, and TGF-β1 were evaluated by Western blot. (d) The expression levels of collagen I, collagen III, and α-SMA were evaluated by IF staining. pcDNA3.1-NC group: HTsFb cells were transfected with pcDNA3.1-NC plasmid; pcDNA3.1-TLR4 group: HTsFb cells were transfected with pcDNA3.1-TLR4 plasmid; sh-NC group: HTsFb cells were transfected with sh-NC plasmid; sh-PAPPA-AS1#1 group: HTsFb cells were transfected with sh-PAPPA-AS1#1 plasmid; group: HTsFb cells were transfected with sh-PAPPA-AS1#1 and pcDNA3.1-TLR4 plasmid simultaneously ( vs. sh-NC, vs. sh-PAPPA-AS1#1).

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