lncRNA PAPPA-AS1 Induces the Development of Hypertrophic Scar by Upregulating TLR4 through Interacting with TAF15

Figure 8

PAPPA-AS1 impacted the fibrosis state of HTS mice by regulating the TAF15/TLR4 axis. (a) The relative expression of TLR4 was detected by qRT-PCR. (b) HE staining was used to evaluate the pathological state of HTS tissues. (c). The expression levels of TLR4, MyD88, and TGF-β1 in the HTS tissues were evaluated by Western blot ( vs. sh-NC, vs. sh-PAPPA-AS1#1). (d) The expression levels of collagen I, collagen III, and α-SMA in the HTS tissues were evaluated by IF staining. pcDNA3.1-NC group: mice were injected with pcDNA3.1-NC lentivirus; pcDNA3.1-TLR4 group: mice were injected with pcDNA3.1-TLR4 lentivirus; sh-NC group: mice were injected with sh-NC lentivirus; sh-PAPPA-AS1#1 group: mice were injected with sh-PAPPA-AS1#1 lentivirus; group: mice were injected with sh-PAPPA-AS1#1 and pcDNA3.1-TLR4 lentivirus simultaneously.

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