Effect of Lipopolysaccharide and TNFα on Neuronal Ascorbic Acid Uptake

Figure 4

Effect of pretreating SH-SY5Y cells with LPS on the activity of SLC23A2 minimal (WT) and mutant promoter constructs. (a) Schematic depiction of SLC23A2 minimal promoter and the locations of Sp1- and KLF-binding sites. (b) SLC23A2 minimal (WT), Sp1- and KLF-binding sites mutated promoter construct activities were determined in LPS-exposed and control SH-SY5Y cells (KLF1 designates the site 5 of the second site named KLF2). Western blot analysis was done to determine the expression levels of Sp1 protein (c), and RT-qPCR was done to determine the level of Sp1 mRNA (d) in LPS-exposed cells. @: cells exposed to LPS treatment significantly decreased compared to KLF1 or KLF2 controls. NS: not significant. Values are of at least 4 separate experiments. ; ; .

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