Migration, Distribution, and Safety Evaluation of Specific Phenotypic and Functional Mouse Spleen-Derived Invariant Natural Killer T2 Cells after Adoptive Infusion

Figure 3

Distribution and metabolism of iNKT cells traced by caliper IVIS Lumina II. (a–c) DiR-labeled spleen-derived iNKT cells were transfused into mice, and the distribution and metabolism (changes in fluorescence intensity) of the iNKT cells were detected. The body surface was monitored from the supine, lateral, and prone positions. (d) Mice were dissected, and organs were isolated and then detected by fluorescence (iNKT cells injected via the tail vein were mainly distributed in the liver and spleen. In vivo detection showed that the fluorescence on body surfaces disappeared at day 34, and in vitro detection showed that the fluorescence of isolated organs disappeared at day 42; (1) thymus, (2) spleen, (3) liver, and (4) groin lymph node). (e) The change in the average fluorescence signal intensity in the spleen and liver.

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