The Key Ingredient Acacetin in Weishu Decoction Alleviates Gastrointestinal Motility Disorder Based on Network Pharmacology Analysis

Figure 6

Acacetin regulated gastrointestinal inflammation and apoptosis. (a) The levels of TNF-α, IL-1β, and IL-6 in serum of mice were determined by ELISA. (b) qRT-PCR was performed to detect the mRNA expressions of inflammatory cytokines TNF-α, IL-1β, and IL-6 and apoptotic proteins Bcl-2, Bax, and Caspase-12. (c) Western blot was used to detect the protein expressions of inflammatory cytokines TNF-α, IL-1β, and IL-6 and apoptotic proteins Bcl-2, Bax, and Caspase-12. CG: control group; MG: model group, LAG: low-dose Acacetin group; MAG: middle-dose Acacetin group; HAG: high-dose Acacetin group; MPG: Mosapride group; vs. CG; ^# vs. MG.

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