Research Article
lncRNA SNHG14 Plays a Role in Sepsis-Induced Acute Kidney Injury by Regulating miR-93
Figure 3
SNHG14 modulates oxidative stress, inflammation, and apoptosis of LPS-induced HK-2 cells via miR-93. (a) Predicted binding site of miR-93 in SHNG14 mRNA. (b) Expression level of miR-93 in LPS-treated HK-2 cells. (C) RIP assays performed using Ago2 antibody exhibited the enrichment of SNHG14 and miR-93. (d) Luciferase assays showed that overexpressing miR-93 decreased the activity of luciferase reporter containing wild-type SNHG14 sequence. (e–p) HK-2 cells were transfected with SNHG14 overexpression plasmids and miR-93 mimics, and LPS-induced HK-2 cells were transfected with SNHG14 shRNAs and miR-93 inhibitor; oxidative stress (e–h), inflammation (i–l), and apoptosis (m–p) were assessed as described above. Data were shown as , .
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