The Inflammasome Signaling Pathway Is Actively Regulated and Related to Myocardial Damage in Coronary Thrombi from Patients with STEMI

Figure 4

Immunohistochemistry staining in thrombi. Histology and immunohistochemistry (brown signal) of thrombus. Initial magnification × 40, but pictures downsized afterwards. Scale bars indicate 50 micrometers. Histologic age (Supplementary Table 1). The panels show the following stainings: HE (a), visualizing fibrin organization and varying infiltration of granulocytes and monocytes. TLR4 (b), present in cytoplasm and cell membrane of monocytes and granulocytes. NLRP3 (c), present in cytoplasm of monocytes. Caspase-1 (d), present in nucleus and cytoplasm of monocytes and granulocytes. IL-1ß (e), present in cytoplasm of monocytes and some granulocytes. IL-18 (f), present in cytoplasm of monocytes and granulocytes. IL-6 (g), present in cytoplasm of monocytes. IL-6R (h), present in cytoplasm of monocytes. Gp130 (i), present in nuclear membrane and cytoplasm of monocytes and granulocytes. Abbreviations: HE: hematoxylin and eosin; TLR4: Toll-like receptor 4; NLRP3: Nod-like receptor protein 3; IL-1β: Interleukin 1β; IL-18: Interleukin 18; IL-6: Interleukin 6; IL-6R: Interleukin 6 receptor; Gp130 = Glycoprotein 130.

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