Omega-3 Supplementation Prevents Short-Term High-Fat Diet Effects on the α7 Nicotinic Cholinergic Receptor Expression and Inflammatory Response

Figure 1

Expression of nicotinic receptors in inflammatory conditions. Chrna7 mRNA levels in the hypothalamus (SC, ; LPS, ) (a); spleen (SC, ; LPS, ) (b); liver (SC, ; LPS, ) (c); and bone marrow (SC, ; LPS, ) (d) of mice after intraperitoneal LPS injection (12 mg/kg or 5 mg/kg of body weight). α7nAChR protein content (SC, ; HFD, ) (e) and Chrna7 mRNA levels (SC, ; HFD, ) (f) in the bone marrow of mice fed a standard chow (SC) or 60% HFD for 3 days (HFD). α7nAChR protein levels in hepatoma cell line Hepa-1c1c7 (C, ; LPS, ) (g) after exposition to LPS (500 ng/mL). The expression of control (GAPDH and β-actin) is shown as percentages (). Student’s -test analysis was used. , , and .

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