Omega-3 Supplementation Prevents Short-Term High-Fat Diet Effects on the α7 Nicotinic Cholinergic Receptor Expression and Inflammatory Response

Figure 4

Expression of nicotinic receptors and severity of sepsis after omega-3 supplementation. α7nAChR protein levels in the (a) hypothalamus (SC, ; HFD, ; HFDω3, ) and (b) bone marrow cells (SC, ; HFD, ; HFDω3, ); (c) Chrna7 mRNA levels in the bone marrow cells (SC, ; HFD, ; HFDω3, ); (d) representative imagens α7nAChR+ cells (α-bgt-green) and F4/80+ cells (red) and nuclear labelling (DAPI-blue) (A—SC, ; B—HFD, ; C—HFD ω3, ); (e) total count of α7nAChR (SC, ; HFD, ; HFDω3, ) and (f) total count of F4-80 (SC, ; HFD, ; HFDω3, ) of mice fed a standard chow (SC) or 60% HFD (HFD) for 3 days and previously supplemented with omega-3 (2 g/kg or 4 g/kg of body weight) for 17 days (HFDω3). (g) p-IKK (SC-SHAM, ; SC-CLP, ; HFD-CLP, ; HFDω3, ), (h) p-NF-κB p65 (SC-SHAM, ; SC-CLP, ; HFD-CLP, ; HFDω3, ) protein content in the hypothalamus, (i) survival rate (SC-SHAM, ; SC-CLP, ; HFD-CLP, ; HFDω3, ), and (j) serum CRP concentration (SC-SHAM, ; SC-CLP, ; HFD-CLP, ; HFDω3, ) of mice fed a standard chow (SC-CLP) or 60% HFD for 3 days (HFD-CLP) and previously supplemented with omega-3 for 17 days (HFDω3-CLP) following CLP surgery. The expression of control (GAPDH and β-actin) is shown as percentages (). In (a, b), Student’s -test analysis was used. In (c, e–h, and j), one-way ANOVA was used. In (i), logrank test was used. Tukey’s HSD test was used for comparison of means. , , and .

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