Omega-3 Supplementation Prevents Short-Term High-Fat Diet Effects on the α7 Nicotinic Cholinergic Receptor Expression and Inflammatory Response

Figure 5

Inflammatory markers in the BV-2 cell line. mRNA transcript levels of Chrna7 (C, ; LPS, ) (a), IL-6 (C, ; LPS, ) (b), IL-1β (C, ; LPS, ) (c), and TNFα (C, ; LPS, ) (d) cytokines in BV-2 cells treated with LPS (100 ng/mL); Western blotting (e) and relative quantification of p-NF-κB p65 (Ser 536) (C, ; LPS, ) (f), DNMT1 (D59A4) (C, ; LPS, ) (g), and ubiquitin (Ub—FL-76) (C, ; LPS, ) (h) in BV-2 cells after LPS treatment (100 ng/mL). The expression of control (GAPDH and β-actin) is shown as percentages (). Student’s -test analysis was used. and .

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