Overexpression of TOLLIP Protects against Acute Kidney Injury after Paraquat Intoxication through Inhibiting NLRP3 Inflammasome Activation Modulated by Toll-Like Receptor 2/4 Signaling

Figure 3

Effect of TOLLIP knockdown on the PQ-induced production of NLRP3 inflammasome in the rat kidney tissues. Wistar rats were infected with the AV carrying shTOLLIP via tail vein injection and then treated with 25 mg/kg PQ via intraperitoneal injection 48 h post-AV infection. (a) The level of IL-18, IL-1β, TNF-α, and IL-6 in the kidney tissues at 24 h post-PQ treatment. (b) The protein expression of NLRP3, ASC, pro-caspase-1, and caspase-1 in the kidney tissues at 24 h post-PQ treatment. (c) Representative images of NLRP3 immunofluorescence staining of the kidney tissues at 24 h post-PQ treatment. and ().

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