Overexpression of TOLLIP Protects against Acute Kidney Injury after Paraquat Intoxication through Inhibiting NLRP3 Inflammasome Activation Modulated by Toll-Like Receptor 2/4 Signaling

Figure 4

Effect of TOLLIP knockdown and overexpression on the PQ-induced loss of cell viability and apoptosis of HK-2 cells. HK-2 cells were infected with the AV carrying shTOLLIP or TOLLIP and then treated with 300 μM PQ 48 h post-AV infection. (a) Cell viability was measured by CCK-8 assay at 24 h post-PQ treatment. (b, c) Cell apoptosis was determined by Annexin V-FITC-PI staining at 24 h post-PQ treatment. (d) The protein expression of TOLLIP, cleaved caspase-3, and cleaved PARP at 24 h post-PQ treatment. , , and ().

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