Overexpression of TOLLIP Protects against Acute Kidney Injury after Paraquat Intoxication through Inhibiting NLRP3 Inflammasome Activation Modulated by Toll-Like Receptor 2/4 Signaling

Figure 5

Effect of TOLLIP knockdown and overexpression on the PQ-induced activation of NLRP3 inflammasome in HK-2 cells. HK-2 cells were infected with the AV carrying shTOLLIP or TOLLIP and then treated with 300 μM PQ 48 h post-AV infection. (a) The protein expression of TLR2, TLR4, NLRP3, pro-caspase-1, caspase-1, and nuclear p65 at 24 h post-PQ treatment. (b) The level of IL-18 and IL-1β at 24 h post-PQ treatment. (c, d) Representative images of p65 and NLRP3 immunofluorescence staining at 24 h post-PQ treatment. and ().

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