Research Article
Glutamine Administration Attenuates Kidney Inflammation in Obese Mice Complicated with Polymicrobial Sepsis
Table 2
Sequences of oligonucleotide primers used for PCR amplification.
| | Gene name | Primer sequence (5→3) | Accession no. |
| | NF-κB | F: TTAGCCAGCGAATCCAGACC | M61909.1 | | R: AGTTCCGGTTTACTCGGCAG | | HMGB-1 | F: CCATTGGTGATGTTGCAAAG | NM_010439.4 | | R: CTTTTTCGCTGCATCAGGTT | | MyD88 | F: CATGGTGGTGGTTGTTTCTGAC | NM_010851.2 | | R: TGGAGACAGGCTGAGTGCAA | | TLR4 | F: AGAAATTCCTGCAGTGGGTCA | NM_021297.2 | | R: TCTCTACAGGTGTTGCACATGTCA | | Kim-1 | F: GCATCTCTAAGCGTGGTTGC | NM_134248.2 | | R: TCAGCTCGGGAATGCACAA | | MCP-1 | F: GATTCACATTTGCGCTGCCT | U12470.1 | | R: TGAGCCTGGGAGATCACCAT | | TNF-α | F: ATGGCCTCCCTCTCATCAGT | NM_013693.3 | | R: TTTGCTACGACGTGGGCTAC | | IL-6 | F: TCCTACCCCAACTTCCAATGCTC | NM_012589.1 | | R: TTGGATGGTCTTGGTCCTTAGCC | | CD68 | F: TGTTCAGCTCCAAGCCCAAA | NM_001291058.1 | | R: ACTCGGGCTCTGATGTAGGT | | EMR-1 | F: ACCTTGTGGTCCTAACTCAGTC | U66889.1 | | R: ACAAAGCCTGGTTGACAGGTA | | ZO-1 | F: GATGTTTATGCGGACGGTGG | BC138028.1 | | R: AAATCCAAACCCAGGAGCCC | | GAPDH | F: AACGACCCCTTCATTGAC | M32599.1 | | R: TCCACGACATACTCAGCAC |
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NF-κB: nuclear factor-κB; HMGB-1: high-mobility group box protein-1; MyD88: myeloid differentiation factor 88; TLR4: toll-like receptor-4; Kim-1: kidney injury molecule-1; MCP-1: monocyte chemoattractant protein-1; TNF-α: tumor necrosis factor-α; IL-6: interleukin-6; CD68: cluster of differentiation 68; EMR-1: epidermal growth factor-like module-containing mucin-like hormone receptor-like-1; ZO-1, zonula occluden-1; GAPDH: glyceraldehyde 3-phosphate dehydrogenase.
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