p300/Sp1-Mediated High Expression of p16 Promotes Endothelial Progenitor Cell Senescence Leading to the Occurrence of Chronic Obstructive Pulmonary Disease

Figure 1

High expression of p16 in EPCs of COPD patients. (a) Peripheral blood EPCs were isolated from nonsmoking non-COPD patients. Flow cytometry verified that the isolated cells were peripheral blood endothelial cells. (b) RT-PCR was used to detect the expression of p16 in peripheral blood EPCs of nonsmoking non-COPD, smoking non-COPD, and smoking COPD patients. (c) Western Blot was used to detect the expression of p16 in peripheral blood EPCs of nonsmoking non-COPD, smoking non-COPD, and smoking COPD patients setting each group with 20 samples. (d) Peripheral blood EPCs from nonsmoking non-COPD patients were treated with different concentrations of CSE, and the expression of p16 was detected by RT-PCR. (e) Peripheral blood EPCs from nonsmoking non-COPD patients were treated with different concentrations of CSE, and the expression of p16 was detected by Western Blot. , , and .

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